FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (23): 172-175.doi: 10.7506/spkx1002-6630-201123034

• Bioengineering • Previous Articles     Next Articles

Optimization of Polypeptide Accumulation Conditions during Soybean Germination

RAO Qing-qing1,YU Xiao-hong1,2,TANG Jun-tao1,HAN Yong-bin1,GU Zhen-xin1,*   

  1. (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Chemistry and Biological Engineering, Yancheng Institute of Technology, Yancheng 224003, China)
  • Online:2011-12-15 Published:2011-12-21

Abstract: Objective: To explore the effect of pH, culture time and culture temperature on polypeptide accumulation in soybean during germination. Methods: Culture conditions optimization for maximizing polypeptide accumulation was performed using response surface methodology based on Box-Behnken experimental design. Results: All the investigated conditions were found to significantly affect the content of polypeptides during the germination process of soybean. Conclusion: The optimal conditions for the accumulation of polypeptides in germinated soybean were achieved after 6 days of culture at 29.0 ℃ in 5 mmol/L, pH 5.2 CBS buffer. The optimized culture resulted in a polypeptide content of 159.38 mg/g soybean, revealing a 3.83-fold increase in compared with non-germinated soybeans.

Key words: soybean, germination, polypeptide, accumulation

CLC Number: