Abstract: In the present study, supercritical CO2 was used to prepare highly acetylated soybean phosphatide from transparent concentrated soybean phospholipid. The optimal preparation conditions were determined as follows: acetic anhydride amount 2.8%, reaction temperature 65 ℃, reaction time 35 min, and reaction pH 7.3. Under these reaction conditions, the acylation rate of soybean phosphatide was 93.2%. Compared with the preparation process at regular state, the amount of acetic anhydride and reaction time were decreased by 0.2% and 5 min, respectively, and the acylation rate was increased by 1.4%. Moreover, an improvement in emulsifying capacity and dispersion capacity was achieved over concentrated soybean phospholipid.

Key words: supercritical CO2, transparent concentrated phospholipid, acetylation, highly acetylated phosphatide