Abstract: A method using [D3]-acrylamide as an isotope internal standard for determining acrylamide in foods was established by solid-phase extraction coupled with high performance liquid chromatography and quadrupole tandem mass spectrometry (HPLC-MS/MS). Analytical procedures were composed of amylase hydrolysis at a concentration of 20 mg/mL, the removal of fat, cleanup by solid-phase extraction (SPE) and the determination by HPLC-MS/MS with a multi-reaction monitoring (MRM) mode and the addition of an internal standard. The limit of detection (LOD) and limit of quantitation (LOQ) of the developed method were 4.8 and 16.5μg/kg, respectively. This method has the advantages of convenient operation, high sensitivity, excellent accuracy and good reproducibility and is therefore suitable for the determination of acrylamide in foods.

Key words: high performance liquid chromatography and quadrupole tandem mass spectrometry (HPLC-MS/MS), solid phase extraction, acrylamide, isotope internal standard