FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (3): 23-27.doi: 10.7506/spkx1002-6630-201203006

• Basic Research • Previous Articles     Next Articles

Enzymatic Hydrolysis Properties and in vitro Digestion of Gorgon Nut (Euryale ferox Salisb.) Starch

ZHANG Cuan,WEI Zhao-jun,YUAN Huai-bo,ZHANG Man   

  1. 1.Department of Chemistry and Life Science, Chuzhou University, Chuzhou 239000, China; 2. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2012-02-15 Published:2012-02-14

Abstract: In order to explore the enzymatic hydrolysis properties of gorgon nut starch, the hydrolysis parameters of gorgon nut starch by α-amylase were optimized. The results showed that the optimal hydrolysis parameters were α-amylase amount of 350 U/g, gorgon nut starch concentration of 10 g/100 mL, hydrolysis pH of 6, hydrolysis temperature of 50 ℃ and hydrolysis time of 60-80 min. Under these conditions, the reducing sugar release from gorgon nut starch was 79.61%. The simulated digestion in vitro showed that the release of reducing sugar and soluble sugar from gorgon nut starch was much lower than that in enzymatic hydrolysis system. Moreover, the digestibility was slightly lower than that of rice starch. Therefore, gorgon nut starch was easily hydrolyzed by α-amylase. The slightly lower digestibility of gorgon nut starch may be related to polyphenols in it.

Key words: gorgon nut starch, α-amylase, enzymatic hydrolysis, simulated digestion

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