Abstract: Flow cytometry and fluorescence microscope were used to explore the effect of Zn2+ on membrane potential and membance permeability in Isochrysis galbana 3011. The processed microalga were detected by flow cytometry and fluorescence microscope with DiBAC4(3) and PI staining through single-factor tests. The statistical software of SPSS 17.0 was used for variance analysis of the data. The results showed that the membrane potential in macrophage was increased significantly and the membance permeability was changed after stimulated with Zn2+ in 5μg/L for 4 min. Therefore, a dynamic membrane status was observed and 5μg/L Zn2+ could lead to partial membrane depolarization and change the membance permeability of Isochrysis galbana 3011, which may be linked to sodium ion channel.

Key words: Isochrysis galbana 3011, Zn2+, flow cytometry, membrane potential, membrane permeability