Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides

doi:10.7506/spkx1002-6630-201205044

Abstract

Abstract: In our present study, response surface methodology was used to optimize process conditions for the hydrolysis of sodium caseinate by trypsin to prepare highly active angiotensin-I converting enzyme (ACE) inhibitory peptides. ACE inhibitory rate was measured by RP-HPLC. One-factor-at-a-time method followed by response surface analysis was used to analyze the effects of pH, temperature, hydrolysis time, substrate concentration, enzyme/substrate ratio on ACE inhibitory activity of sodium caseinate hydrolysate. A mathematical model describing the relationship of ACE inhibitory activity of sodium caseinate hydrolysate with temperature (A), hydrolysis time (B), substrate concentration (C) and enzyme/substrate ratio (D) was obtained as follows: Y=－11.21347＋4.32902A－1.45953B＋3.42928C－0.20303D＋0.050303AB＋0.047422AD＋0.14955BC ＋0.12486BD － 0.054526A2 － 0.079754B2 － 0.53587C2 － 0.28096D2. The optimum hydrolysis conditions were determined as pH 7.0, 52.31 ℃, 19.44 h, substrate concentration of 5.91 g/100 mL and enzyme/substrate ratio of 8.37‰, resulting in an ACE inhibitory rate of 97.11%.

Key words: sodium caseinate, trypsin, ACE inhibiting peptides, response surface methodology, RP-HPLC

CLC Number:

TS218

XU Xin，CAI Li-li，LIU Guo-yan，HE Jia-yi，WEI Xiao-rui，Wang Zhi-ying，ZHANG Jun. Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides[J]. FOOD SCIENCE, 2012, 33(5): 208-212.

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Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides

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