Separation, Purification and Structural Identification of Small Peptides from Jinhua Ham

doi:10.7506/spkx1002-6630-201209004

Abstract

Abstract: Tangential flow filtration (TFF) and Sephadex G-15 gel filtration chromatography were used for the separation and purification of low molecular weight polypeptides in Jinhua ham at the end of sun-drying and post-ripening. Twenty-six main peptides were obtained and analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Seven of them had better repeatability and higher relative peak area. The amino acid sequences were Ala-His, Pro-Leu, Ser-Gly-Val, Ser-Gly-Phe, Pro-Leu-Lys-Pro-Ala, Leu-Leu-Ala-His and Asn-Pro-Thr, and the relative peak areas 17.81%, 8.62%, 10.58%, 5.70%, 6.11%, 6.12% and 3.49%, respectively.

Key words: jinhua ham, small peptide, separation and purification, LC-MS/MS, amino acid sequence

CLC Number:

TS251.6

WANGJuan，ZHAOGai-ming，ZHANGJian-wei，LIUYan-xia，LIMiao-yun，HUDong-hua. Separation, Purification and Structural Identification of Small Peptides from Jinhua Ham[J]. FOOD SCIENCE, 2012, 33(9): 16-20.

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Separation, Purification and Structural Identification of Small Peptides from Jinhua Ham

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