Extraction, Purification and Properties of Pumpkin Peel Polysaccharide PP1

doi:10.7506/spkx1002-6630-201209026

Abstract

Abstract: A polysaccharide from pumpkin peel, named as PP1, was extracted by hot water extraction method and purified by cetyltrim-ethlammonium bromide (CTAB) method. UV spectroscopy and HPLC were both used to analyze its purity, and its structure and monosaccharide composition were analyzed by IR spectroscopy and GC, respectively. The microstructure of PP1 was observed under an AFM based on fixation with ethanol or Ni2+. The results showed that PP1 was highly pure and contained neither nuclear acid nor protein. IR spectroscopic analysis indicated that PP1 had characteristic absorption peaks of polysaccharides and pyran ring. Its monosaccharide composition was composed of fructose, arabinose, glucose and galactose at a molar ratio of 0.92:2.76:5.52:2.24. AFM observations showed that its structure was a single chain with no branches. PP1 had a long thin structure when fixed with ethanol but a short fat structure when fixed with Ni2+.

Key words: pumpkin peel polysaccharide PP1, atomic force microscope, structural analysis

CLC Number:

TQ929.2

BAIDi-si，MADong，RUIQian，ZHANGJing. Extraction, Purification and Properties of Pumpkin Peel Polysaccharide PP1[J]. FOOD SCIENCE, 2012, 33(9): 122-126.

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Extraction, Purification and Properties of Pumpkin Peel Polysaccharide PP1

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