Purification and Structural Analysis of a Polysaccharide from Bitter Gourd (Momordica charantia)

doi:10.7506/spkx1002-6630-201510018

Abstract

Abstract:

In this research, a water-soluble polysaccharide (MCP Ⅱa) was extracted from Momordica charantia by cellulase
hydrolysis, precipitated with ethanol, and then purified by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel
filtration chromatography. The high performance gel permeation chromatography (HPGPC) analysis showed that the average
molecular weight of MCP Ⅱa was 13.0 kD. Monosaccharides analysis revealed that the MCP Ⅱa was composed of Rha,
GalA, Gal, Xyl and Ara with a relative molar ratio of 12:3.05:19.89:5.95:56. Infrared (IR) spectrum, 13C-nuclear magnetic
resonance (NMR) and Cango-red tests indicated that MCP Ⅱa formed a β-triple helix in aqueous solution and the C1, C2
and C3, C5 positions were its binding sites. Moreover, it existed as a rhombus crystal under a scanning electron microscope.

Key words: Momordica charantia, polysaccharide, purification, structural analysis

CLC Number:

O629.12

CHEN Hongman, GUO Longwei, ZHANG Shaozai, LIU Jiangman, ZHANG Haiyan, KAN Guoshi*. Purification and Structural Analysis of a Polysaccharide from Bitter Gourd (Momordica charantia)[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201510018.

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Purification and Structural Analysis of a Polysaccharide from Bitter Gourd (Momordica charantia)

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