Abstract: Actinidia arguta was used as the experimental material to explore the optimal extraction conditions and enzymatic activity analysis conditions of polygalacturonase (PG). The optimal extraction process parameters were explored by orthogonal tests through evaluating the effects of pH, ion concentration and DTT addition amount on extraction rate PG. The results indicated that the optimal extraction process condtions for PG were extraction pH of 5.5, acetate buffer concentration of 0.05 mol/L, NaCl concentration of 0.1 mol/L and DTT concentration of 1 mmol/L. The optimal enzymatic activity analysis conditions were reaction temperature of 40 ℃ and reaction time of 90 min.

Key words: Actinidia arguta, polygalacturonase (PG), extraction, enzymatic activity analysis