Abstract: This paper describes a method to determine benzo(a) pyrene in animal fats and vegetable and oils using gel permeation-high performance liquid chromatography with fluorescence detector (HPLC-FLD). In this method, gel permeation cleanup system was used to remove the interference such as triglyceride. Chromatographic separation was achieved by a reverse-phase column of Hypersil ODS2 with the mobile phase consisting of methanol-water (94:6, V/V) at a flow rate of 1.0 mL/min. FLD were set at 365 nm excitation and 406 nm emission wavelengths, and the column temperature was kept at 30 ℃. The limit of detection was 0.1μg/kg, the calibration curve was linear between a concentration range of 0.1－50μg/kg, the recoveries were in the range of 92.0%－106.0%, and RSD (n ＝ 6) value was 1.86%. The method is simple, rapid, accurate and sensitive for directly determining benzo(a)pyrene in animal fats and vegetable oils without complicated and time-consuming separation process.

Key words: benzo(a)pyrene, GPC cleanup system, high performance liquid chromatography (HPLC), fluorescence detection (FLD), animal fats and vegetable oils