Abstract: Nucleosides extraction from rape bee pollen was optimized using response surface methodology. The appropriate ranges of extraction time, extraction temperature and material-to-liquid ratio were obtained by one-factor-at-a-time experiments for subsequent optimization using response surface analysis based on a three-variable, three-level central composite design. The optimal ranges of extraction time, extraction temperature and material-to-liquid ratio were 40－60 min, 45－65 ℃ and 1:60－1:80 (g/mL), respectively. The optimized extraction conditions were 53 min, 60 ℃, and 1:70 (g/mL). Under these conditions, the extraction rate of nucleosides from rape bee pollen was (705.2 ±0.006) mg/100 g.

Key words: response surface methodology, nucleosides, rape bee pollen, Box-Behnken design, ultrasonic extraction, optimization