High-Speed Shear Treatment for Cell Wall Disruption of Rape Bee Pollen

doi:10.7506/spkx1002-6630-201212019

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (12): 97-101.doi: 10.7506/spkx1002-6630-201212019

• Processing Technology • Previous Articles Next Articles

High-Speed Shear Treatment for Cell Wall Disruption of Rape Bee Pollen

LI Li1,2，LIU Ye-wei1,*，ZHAO Jian-xi1,2，PEI Dong2,3，DI Duo-long2，WANG Na1

(１. Institute of Nutrition and Food Hygiene, College of Public Health, Lanzhou University, Lanzhou 730000, China； 2. Key Laboratory of Chemistry of Northwestern Plant Resources Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China； 3. Graduate University of Chinese Academy of Sciences, Beijing 100039, China)

Online:2012-06-25 Published:2012-07-27

Abstract

Abstract: A high-speed shear dispersing emulsifier (HSDE) was used to treat rape bee pollen for cell wall disruption. The effects of treatment time, temperature, HSDE speed and ratio of raw material to liquid on disruption rate in aqueous medium were investigated. A predictive polynomial quadratic model was set up by means of central composite design and used to optimize these treatment conditions by response surface methodology. A scanning electron microscope (SEM) was used to characterize pollen morphology, and total flavonoids content was determined by colorimetric method to further explain the effectiveness of HSDE treatment in cell wall disruption. The optimal HSDE treatment conditions were determined as 3 min of treatment time, 22000 r/min of HSDE rotary speed, and 1:3 of material-to-liquid ratio (mL/g), resulting in a disruption rate exceeding 99% and a flavonoid yield of 3.60 mg/g. In conclusion, HSDE treatment is a good pretreatment strategy that can provide a high disruption rate in a short time.

Key words: rape bee pollen, break cells, high-speed shear dispersing emulsifier, response surface methodology

CLC Number:

TS201.1

LI Li，LIU Ye-wei，ZHAO Jian-xi，PEI Dong，DI Duo-long，WANG Na. High-Speed Shear Treatment for Cell Wall Disruption of Rape Bee Pollen[J]. FOOD SCIENCE, 2012, 33(12): 97-101.

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High-Speed Shear Treatment for Cell Wall Disruption of Rape Bee Pollen

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