Ultrasonic-Assisted Extraction and Bioactivity of Polysaccharides from Pickled Undaria pinnatifida

doi:10.7506/spkx1002-6630-201224021

Abstract

Abstract:

The ultrasonic-assisted extraction of polysaccharides from pickled Undaria pinnatifida (PUP) was optimized using an orthogonal array design, and the antioxidant and antibacterial activities of PUP were investigated. The optimum conditions for PUP extraction were found to be two extraction cycles at 70 ℃ for 10 min each cycle with an ultrasonic power of 180 W and a solid-to-solvent ratio of 1:40 (g/mL). Under these conditions, the PUP yield was 2.00%. Crude PUP was purified by Sevag method for the removal of impurities such as protein and nuclei acid. UV and infrared spectroscopic characterization suggested that purified PUP was polysaccharide without containing protein or nuclei acid. The scavenging activity of purified PUP against hydroxyl and superoxide anion radicals was investigated and compared with VC as a control. It was found that purified PUP had strong scavenging activity against both species of free radicals, especially superoxide anion radical although its scavenging activity was weaker than that of VC. The IC50 values of PUP against superoxide anion and hydroxyl free radicals were 16.50 mg/mL and 23.31 mg/mL, respectively. Moreover, PUP had potent antibacterial activity against Staphylococcus aureus, Escherichia coli and Bacillus subtilis.

Key words: pickled Undaria pinnatifida, polysaccharides, ultrasonic-assisted extraction, antioxidant activity, antibacterial activity

CLC Number:

R284.2

References

[1] GUPTA S, ABU-GHANNAM N. Bioactive potential and possible health effects of edible brown seaweeds[J]. Trends in Food and Technology, 2011, 22: 315-326.

[2] MOHAMED S, HASHIM S N, RAHMAN H A. Seaweeds: a sustainable functional food for complementary and alternative therapy[J]. Trends in Food and Technology, 2011, in press.

[3]AL-ALAWI A, AL-MARHUBI I M, AL-BELUSHI M S M, SOUSSI B. Characterization of carrageenan extracted from Hypnea bryoides in Oman[J]. Marine Biotechnology, 2011, doi 10.1007/s10126-010-9350-7

[4] 谭仁祥．功能海洋生物分子——发现与应用[M]．北京：科学出版社．2007,1.

[5] 阮积惠. 海藻主要药用成分的研究和展望[J]. 东海海洋, 2001, 19(2): 1-9.

[6] LIN X P, ZHENG Y, CHEN X Q. A review of antimicrobial substances of marine algae[J]. Subtropical Plant Science, 2008, 37(3): 76-80.

[7] HOLDT S L, KRAAN S. Bioactive compounds in seaweed: functional food applications and legislation[J]. Journal of Applied Phycol. 2011, Doi 10.1007/s10811-010-9632-5.

[8] ZHANG Z S, WANG F, WANG X M, et al. Extraction of the polysaccharides from five algae and their potential antioxidant activity in vitro[J]. Carbohydrate Polymers, 2010, 82: 118-121.

[9] 罗先群, 王新广, 杨东升. 海藻多糖的结构、提取和生物活性研究进展[J]. 中国食品添加剂, 100-105.

[10] ANANTHI S, RAGHAVENDRAN H R B, SUNIL A G, et al. In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (marine brown alga) [J]. Food and Chemical Toxicology, 2010, 48: 187-192.

[11] SUETSUNA K, MAEKAWA K, CHEN J R. Antihypertensive effects of Undaria pinatifida (wakame) peptide on blood pressure in spontaneously hypertensive rats[J]. Journal of Nutritional Biochemistry, 2004, 15: 267-272.

[12] 张文竹, 刘红兵, 管华诗. 裙带菜的化学成分及其生物活性研究进展[J]. 海洋科学, 2009, 33(4): 72-75.

[13] 王彩, 施松善, 王顺春. 裙带菜多糖研究进展[J]. 时珍国医国药, 2010, 21(1): 206-208.

[14] VISHCHUK O S, ERMAKOVA S P, ZVYAGINTSEVA T N. Sulfated polysaccharides from brown seaweeds Saccharina japonica and Undaria pinnatifida: isolation, structural characteristics, and antitumor activity[J]. Carbohydrate Research, 2011, doi:10.1016/j.carres.2011.09.034.

[15] 朱良, 王一飞, 朱艳梅. 裙带菜孢子叶多糖含量的测定[J]. 食品科学, 2005, 26(10): 184-186.

[16] 朱良, 张青, 朱钦昌, 等. 正交设计优化裙带菜孢子多糖提取工艺[J]. 中国海洋药物杂志, 2006, 25(6): 42-45.

[17] SYNYTSYA A, KIM W J, KIM S M, et al. Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida[J]. Carbohydrate Polymers, 2010, 81: 41-48.

[18] 李巧云, 居红芳, 翟春. 五味子粗多糖提取工艺的研究[J]. 食品科学, 2004, 25(5): 105-109.

[19] 孙杰, 王艳杰, 朱路英, 等. 石花菜醇提物抑菌活性和抗氧化活性研究[J]. 食品科学, 2007, 28(10):53-56.

[20] 吐尔干乃义?吐尔逊, 热衣木?马木提, 阿不都拉?阿巴斯. 中国树花中地衣多糖的抗氧化活性研究[J]. 食品与发酵工业, 2009, 35(11): 119-121.

[21] WU Y, CUI S W, TANG J. et al. Preparation, partial characterization and bioactivity of water-soluble polysaccharides form boat-fruited sterculis seeds[J]. Carbohydrate Polymer, 2007, 70: 437-443.

[22] ANANTHI S, TAGHAVENDRAN H R B, SUNIL A G, et al. In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (marine brown agla)[J]. Food and Chemical Toxicology, 2010, 48: 187-192.