Abstract:

The preparation of inclusion complexes of Perilla frutescens seed oil with β-cyclodextrin using a microfluidizer to increase the α-linolenic acid content in the oil was studied. Response surface methodology based on a Box-Behnken design was used to optimize Perilla frutescens seed oil-to-β-cyclodextrin ratio, pressure and inclusion reaction time. The maximum inclusion rate as high as 81.64% was obtained after 105 min of inclusion reaction between Perilla frutescens seed oil and  β-cyclodextrin at a ratio of 1:11 (mL/g) at 6000 psi. FT-NIR analysis and SEM observation showed that Perilla frutescens seed oil was well included by β-cyclodextrin in the inclusion complex obtained. The α-linolenic acid content increased from 53.50% to 77.42% after β-cyclodextrin inclusion as shown by HPLC analysis. Therefore, β-cyclodextrin inclusion is a feasible way to concentrate the α-linolenic acid content in Perilla frutescens seed oil.

Key words: homogenization, inclusion, Perilla frutescens seeds, α-linolenic acid, response surface methodology