Optimized Synthesis of Glucose Palmitate by Immobilized Lipase from Candida sp. with Response Surface Methodology

doi:10.7506/spkx1002-6630-201306005

Abstract

Abstract: The effects of lipase addition amount, substrate concentration, palmitic acid-glucose ratio, reaction temperature, reaction time, and 4 ? molecular sieve amount on the esterification of glucose were studied. The optimal ranges of independent variables were selected on the basis of single-factor tests. Response surface methodology and central composite rotatable design were adopted to evaluate the effects of synthesis variables on the degree of esterification. The results showed that reaction time was the most important parameter. The optimal conditions for esterification were lipase addition amount of 14.67 U, substrate concentration of 1.31 mol/L, palmitic acid-glucose ratio of 3.03:1, reaction time of 6.63 h, 4 ? molecular sieve amount of 0.71 g, and reaction temperature of 45 ℃. The actual degree of esterification was 89.72%, which was close to the predicted value of 94.55%. After seventh repeated use, the residual activity of immobilized lipase remained 40%.

Key words: immobilized Candida sp. Lipase, response surface, optimization, sugar esters, esterification

CLC Number:

References

[1]YOO I S, PARK S J, YOON H H.Enzymatic synthesis of sugar fatty acid esters[J].J Ind Eng Chem,2007,13(1):1-6
[2]COULON D., ISMAIL A, GIRARDIN M, et al. Enzymatic synthesis of alkylglycoside fatty acid esters catalyzed by an immobilized lipase[J].. J Mol Catal B: Enzym,1998,5(1-4):45-48
[3]VILLENEUVE P, MUDERHWA J M, GRAILLE J.et alCustomizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches[J].. J Mol Catal B: Enzym,2000,9(4-6):113-148
[4]YAN Y, BORNSCHEUER U T, STADLER G, et al.Production of sugar fatty acid esters by enzymatic esterification in a stirred-tank membrane reactor: optimization of parameters by response surface methodology[J].J Am Oil Chem Soc,2001,78(2):147-153
[5]SHIEH C, CHANG S W.Optimized synthesis of lipase-catalyzed hexyl acetate in n-hexane by response surface methodology[J].,2001,49(3):1203-1207
[6]YU J, ZHANG J, ZHAO A, et al.Study of glucose ester synthesis by immobilized lipase from Candida sp[J].. Catal Commun,2008,9(6):1369-1374
[7]NETA N S, PERES A M, TEIXEIRA J A, et al.Maximization of fructose esters synthesis by response surface methodology[J].New Biotechnol,2011,28(4):349-355
[8]MURALIDHAR R V, CHIRUMAMILA R R, MARCHANT R, et al.A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources[J].Biochem Eng J,2001,9(1):17-23
[9]Hari-KRISHNA S.nullMANOHAR B, DIVAKAR S, et al. Lipase-catalyzed synthesis of isoamyl butyrate: optimization by response surface methodology[J].J Am Oil Chem Soc,1999,76(12):1483-1488
[10]SHAW J F, WU H Z, SHIEH C J.Optimized enzymatic synthesis of propylene glycol monolaurate by direct esterification[J].Food Chem,2003,81(1):91-96
[11]MELO L L M M, PASTORE G M, MACEDO G A.Optimized synthesis of citronellyl flavour esters using free and immobilized lipase from Rhizopus sp[J].. Process Biochem,2005,40(10):3181-3185
[12]CHELLAPANDIAN M.nullSASTRY C A. Immobilization of alkaline protease on nylon[J].Bioproc Biosyst Eng,1994,11(1):17-21
[13]ZAIDI A, GAINER J L, CARTA G.Fatty acid esterification using nylon-immobilized lipase[J].Biotechnol Bioeng,1995,48(6):601-605
[14]ABEDER S, HABULIN M, KNEZ ?.Lipase-catalyzed synthesis of fatty acid fructose esters[J].J Food Eng,2006,77(4):880-886
[15]COCHRAN W G, COX G M.[J].Experimental Designs[M]. New York: John Wiley and Sons,1992,:-
[16]WEI D, GU C, SONG Q, et al.Enzymatic esterification for glycoside lactate synthesis in organic solvent[J].Enzyme Microb Technol,2003,33(4):508-512
[17]DUAN Y, DU Z, YAO Y, et al.Effect of molecular sieves on lipase-catalyzed esterification of rutin with stearic acid[J].J Agric Food Chem,2006,54(17):6219-6225
[18]KIM B H, AKOH C C.Modeling and optimization of lipase-catalyzed synthesis of phytosteryl esters of oleic acid by response surface methodology[J].Food Chem,2007,102(1):336-342
[19]CHEN X, DU W, LIU D.Response surface optimization of biocatalytic biodiesel production with acid oil[J].Biochem Eng J,2008,40(3):423-429