Effect of Long-Term Intervention with Proanthocyanidin on Oxidative Stress in Type Ⅱ Diabetes

doi:10.7506/spkx1002-6630-201307055

Abstract

Abstract: Objective: To observe the oxidative stress in Type Ⅱ diabetic rats after long-term feeding of grape seed proanthocyanidin extract (GSPE) and the effect of GSPE intervention on oxidative stress. Methods: Forty-eight Type Ⅱ diabetic rats that were successfully induced were randomly divided into 4 groups: three GSPE-treated groups and one diabetic model group. Twelve rats fed on normal forage were served as normal control group. GSPE-treated groups were administered with GSPE by gavage at doses of 125, 250, 500 mg/(kg·d). Diabetic model group and normal control group were administered with deionized water also by gavage. After 24 weeks, all rats were killed and blood and organs were acquired. Oxidative indicators (SOD, GSH-Px and MDA) of all tissues were detected. Results: Compared with the normal control group, SOD activity in the serum and liver tissue of the diabetic model group was significantly decreased, so was the GSH-Px activity in liver and muscle tissue, but the amount of MDA in the brain of the diabetic model group was increased; compared with the diabetic model group, SOD activity in the serum, liver and spleen and GSH-Px activity in the serum, liver, muscle and spleen from the intervention groups was significantly increased (P ＜ 0.05). Conclusion: The level of oxidative damage in different tissues from Type Ⅱ diabetic rats is different. Long-term intervention with GSPE can reduce oxidative damage to tissues.

Key words: grape seed proanthocyanidin extract, Type Ⅱ diabetes, long-term feeding, superoxide dismutase, glutathione peroxidase, malonaldehyde

CLC Number:

151.2

JIANG Yan-fei，ZHANG Zhao-feng，BAO Lei，JING Lu-lu，DING Ye，DAI Xiao-qian，MA Xiao-tao，LI Yu-jie，CAI Xia-xia，LI Yong*. Effect of Long-Term Intervention with Proanthocyanidin on Oxidative Stress in Type Ⅱ Diabetes[J]. FOOD SCIENCE, 2013, 34(7): 262-265.

References

[1]http://www.diabetes.org/diabetes-basics/diabetes-statistics/.[OL].
[2]Bagchi D,Garg A,Kmhn RL,et al. Oxygen free radical scavenging abilities of vitamin C and vitamin E,and a grape seed proanthocyanidins extract in vitro[J]. Research Communications in Molecular Pathology and Pharmacology,1997, 95(2): 179-189.
[3]Brownlee M.The pathobiology of diabetic complications: a unifying mechanism[J]. Diabetes,2005,54(6):1615-1625.
[4]Brownlee M. Biochemistry and molecular cell biology of diabetic complications[J].Nature, 2001,414(6865):813-820.
[5] Prasenjit M, Jyotirmoy G, Joydeep D, et al. Streptozotocin induced activation of oxidative stress responsive splenic cell signaling pathways: Protective role of arjunolic acid[J]. Toxicology and Applied Pharmacology,2010,244(2):114-129.
[6] Geerlings, S.E., Hoepelman, A.I..Immune dysfunction in patients with diabetes mellitus (DM)[J]. FEMS Immunology & Medical Microbiology, 1999,26:259-265.
[7] Chalasani N, Deeg M, Crab D. Systemic levels of lipid peroxidation and its metabolic and dietary correlates in patients with non-alcoholic steatohepatitis[J].Am J Gastroenterol 2004,99:1497-1502.
[8] Yesilova Z,Yaman H,Oktenli C,Ozcan A,Uygun A, et al. Systemic markers of lipid peroxidation and antioxidants in patients with non-alcoholicfatty liver disease[J]. Am J Gastroenterol. 2005,100:850-855.
[9]Roberta S, Luciane B. P, Naiara S, et al.Aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic rats [J].Protective role of arjunolic acid Toxicology and Applied Pharmacology. 2010 ,244: 114–129.
[10]Schulman IH,Zhou MS.Vascular insulin resistance:a potential link between cardiovascular and metabolic diseases[J]. Curr Hypertens Rep.2009,11:48–55.
[11] Gumieniczek H,Hopka?a Z, Wojtowicz M,et al.Differences in antioxidant status in skeletal muscle tissue in experimental diabetes [J].Clinica Chimica Acta. 2001, 314. 39–45 .
[12]Biessels, G.J., van der Heide, L.P., Kamal A.et al. Ageing and diabetes: implications for brain function[J]. Eur. J. Pharmacol. 2002,441:1-14.
[13]Siddiqui,M.R., Taha, A., Moorthy, K.et al. Amelioration of altered antioxidant status and membrane linked functions by vanadium and Trigonella in alloxan diabetic rat brains[J]. Biosci. 2005, 30:483–490.
[14]Li M, Ma Y, Gao H, Li B, et al. A novel approach of proteomics to study the mechanism of action of grape seed proanthocyanidin extracts of diabetic retinopathy in rats[J]. Chinese Medical Journal. 2008, 121(24): 2544-2552.
[15]Li B, Cheng M, Gao H, Ma Y, et al. Back-regulation of six oxidative stress proteins with grape seed proanthocyanidin extracts in rat diabetic nephropathy[J]. Journal of Cellular Biochemistry. 2008, 104(2): 668-679.
[16]Lu M, Xu L, Li B, Zhang W, et al. Protective effects of grape seed proanthocyanidin extracts on cerebral cortex of streptozotocin-induced diabetic rats through modulating AGEs/RAGE/NF-κB pathway[J]. Journal of Nutritional Science and Vitaminology. 2010, 56 (2): 87-97.
[17]Pinent M, Blay M, Blade MC, et al. Grape seed-derived procyanidins have an anti-hyperglycemic effect in streptozotocin-induced diabetic rats and insulin mimetic activity in insulin-sensitive cell lines[J]. Endocrinology.2004,145(11):4985-4990.
[18]Li X, Li B, Gao H, Cheng M, et al. Effects of grape seed proanthocyanidin extracts on aortic pulse wave velocity in streptozocin induced diabetic rats[J]. Bioscience, Biotechnology, and Biochemistry. 2009, 73(6): 1348-1354.
[19] LI Lan fang, LI Jian .Link between oxidative stress and insulin resistance [J]. Chinese medical sciences journal,2007,22（4）:254-259.
[20] Sarah A, Srikanth B,Helen RG. Dietary antioxidant interventions in type 2 diabetes patients: a meta-analysis［J］.British Journal of Diabetes & Vascular Disease，2011,11（2）:62-68.