Abstract:

A method for the determination chloranteraniliprole and flubendiamide residues in animal-origin foodstuffs
using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. Residues of chloranteraniliprole
and flubendiamide in animal-origin foodstuffs were extracted with a mixture of acetonitrile and ammonium acetate and
decontaminated on a neutral alumina column using acetonitrile and aqueous solution containing 5 mmol/L ammonium
acetate (70:30) as mobile phase. LC-MS/MS analysis of the analytes was conducted by electrospray ionization (ESI) in a
positive mode using multiple reaction monitoring, and quantified by an external standard method. The results showed that the
detection limit was 1 μg/kg for both chloranteraniliprole and flubendiamide. An excellent linear relationship was observed in the
range of 1 to 250 μg/L with a relative standard deviation of 0.999. The recovery rates of chloranteraniliprole and flubendiamide
at spiked levels of 1, 2 μg/kg and 10 μg/kg were 73.5%–101.2% with relative standard deviation of 3.72%–11.05%.

Key words: animal-origin foodstuffs, chloranteraniliprole, flubendiamide, liquid chromatography-tandem mass spectrometry (LC-MS/MS), determination