Abstract:

To explore the defecating function of fructooligosaccharide (FOS) and Lactobacillus, constipation model of
Wistar rats was established. From 36 male wistar rats of the same age and weight, 4 rats were randomly selected as blank
control group, while the remaining rats were used as constipation model and randomly divided into 8 groups, including
model group, positive treatment group (folium senna 10.0 mg/kg), FOS high, medium and low-dose groups (corresponding
to 1.0, 0.5, 0.25 g/(kg·d) and Lactobacillus high, medium and low-dose groups(corresponding to 2.0 × 1010, 2.0 × 109,
2.0 × 108 CFU/(kg·d)). After continuous force-feeding for 7 days, 6 indexes, i.e., feed intake, water intake, body mass
increment, the advance rate of carbon powder, fecal pellets and intestinal flora, were detected. Both FOS and Lactobacillus
had defecating function. Water intake, body mass increment, the advance rate of carbon powder and fecal pellets of the
6 groups with gavage of FOS or Lactobacillus were significantly higher than those of model group (P ＜ 0.05). Besides,
FOS and Lactobacillus had an inhibiting effect on the growth of intestinal Enterococci and Enterobacteriaceae (P ＜
0.05). The optimal dosage of FOS and Lactobacillus was 1.0 g/(kg·d) and 2.0 × 108 CFU/(kg·d), respectively. Both FOS
and Lactobacillus have defecating function, maintain the balance of intestinal flora and inhibit the growth of intestinal
Enterococci and Enterobacteriaceae.

Key words: fructooligosaccharides, Lactobacillus, constipation, laxative effect, Wistar rat