Purification of Phytosterol Oxidation Products in the Lipid Matrix

doi:10.7506/spkx1002-6630-201314002

Abstract

Abstract:

Abstract：Lipid matrix was saponified for the purification of phytosterol oxidation products from it. Response surface
methodology was applied to optimize four process conditions based on phytosterol concentration. A mathematical model
was developed indicating the effect of the studied variables on phytosterol concentration. Results showed that the optimal
saponification parameters were addition of 40.04 mL/g of 3.22 mol/L KOH ethanol solution, 69.07 ℃ and 2.02 h. The final
product obtained under the optimized conditions contained 0.1455 mg/mL phytosterols.

Key words: lipid matrix, phytosterols, saponification, response surface methodology, purification

CLC Number:

TS221

JIANG Shao-tong，PAN Ying，PANG Min，PAN Li-jun，CAO Li-li. Purification of Phytosterol Oxidation Products in the Lipid Matrix[J]. FOOD SCIENCE, 2013, 34(14): 6-11.

References

[1]庞敏, 姜绍通, 潘丽军, 等. 不同加热方式对脂质基质中甾醇氧化稳定性影响[J].食品科学,0,():0-0.

[2]LAMPI A M, JUNTUNEN L, TOIVO J, et al.Determination of thermo-oxidation products of plant sterols[J].Journal of Chromatography B,2002,777:83-92.

[3]FORD A, MAGUIRE L, KONOPLYANNIKOV M, et al.Comparison of the cytotoxic effects of beta-sitosterol oxides and a cholesterol oxide, 7beta-hydroxycholesterol, in cultured mammalian cells[J].British Journal of Nutrition,2003,90:767-775.

[4]BAKER V A, HEPBURN P A, KENNEDY S J, et al.Safety evaluation of phytosterol esters, Part IAssessment of oestrogenicity using a combination of in vivo and in vitro assays[J].. Food and Chemical toxicology,1999,37:13-19.

[5]BERGER A, JONES P H J, ABUMWEIS S S.Plant sterols: factors affecting their efficacy and safety as functional food ingredients[J].,2004,3:5.

[6]KIM J C, KANG B H, SHIN C C, et al.Subchronic toxicity of plant sterol esters administered by gavage to Sprague-Dawley rats[J].Food and Chemical toxicology,2002,40:1569-1580.

[7]RUDZINSKA M, PRZYBYLSKI R, et al.Sitosterol Thermo-oxidative Degradation Leads to the Formation of Dimers, Trimers and Oligomers: A Study Using Combined Size Exclusion Chromatography / Mass Spectrometry[J].Lipids,2010,45(6):549-558.

[8]GARCIA-LIATAS G, RODRIGUEZ-ESTRADA M T.Current and new insights on phytosterol oxides in plant sterol-enriched food[J].Chemistry and Physics of Lipids,2011,164(6):607-624.

[9]PORTER N A, CALDWELL S E and Mills K A.Mechanisms of free radical oxidation of unsaturated lipids[J].Lipids,1995,30:277-290.

[10]DUTTA P C. Chemistry，analysis, and occurrence of phytosterol oxidation products in foods[M]// DUTTA P C. Phytosterols as functional food components and nutraceuticals.[J].New York: Marcel Dekker Inc,2004,:397-417.

[11]庞敏, 姜绍通.甾醇氧化特性及其在食品中应用研究进展[J].食品科学,2010,31(23):434-438.

[12]BUSCH Y P, KING A J.Stability of Cholesterol, 7-Ketocholesterol and β-Sitosterol during Saponification: Ramifications for Artifact Monitoring of Sterol Oxide Products[J].J Am Oil Chem Soc,2010,87(9):955-962.

[13]SODEIF A D, DUTTA P C.A single step solid-phase extraction method for complete separation of sterol oxidation products in food lipids[J].J Chromatograp A,2009,1216(1):36-42.

[14]倪芳妍，刘配莲，孟橘, 等.植物油中总甾醇测定前处理方法的研究[J].中国油脂,2011,36(2):74-76.

[15]曹智.从天然维生素E生产渣油中提取植物甾醇皂化工艺的研究[J].中国油脂,2010,35(4):54-56.