Abstract:

The process conditions for microwave-assisted extraction of salidroside from Rhodida rosea were optimized.
HPLC was used to detect salidroside. The effects of seven process parameters including material particle size, microwave
treatment time, microwave power, alcohol concentration, extraction temperature, extraction time and solid-liquid ratio on
the extraction rate of salidroside were investigated through single factor tests. The significance and interaction effects of
microwave treatment time, microwave power, ethanol concentration and extraction temperature were analyzed by response
surface analysis based on a three-level central composite design. The optimal microwave-assisted extraction conditions for
salidroside were achieved to be microwave treatment duration of 42 s, microwave power of 480 W, ethanol concentration of
78%, solid-liquid ratio of 1:10 (g/mL) and extraction temperature of 72 ℃, extraction time of 90 min and material particle
size of 40 mesh. Under these extraction conditions, the extraction rate of salidroside from Rhodida rosea was up to 93.53%.

Key words: salidroside, extraction process, optimization, response surface methodology