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Microbiological Causes of Swollen Bag in Pork Jelly Analyzed by 16S rDNA and PCR-DGGE

GAO Peng,WANG Yan,HUANG Min,CHEN Hao,SUN Qun   

  1. 1. Sichuan Institute of Atomic Energy, Chengdu 610101, China;2. Key Laboratory for Bio-resources and Eco-environment,Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China
  • Online:2013-07-25 Published:2013-08-02

Abstract:

Swollen bag can affect the quality of vacuum packaged meat products. The causes of swollen bag in pork
jelly were studied. Totally 14 and 18 stains isolated from the control group and package swollen pork jelly group were
identified by 16S rDNA analysis, and PCR-DGGE was also used to analyze the bacterial community in the two groups.
According to the results, some bacteria were detected in the package swollen pork jelly group, but were not found in
the control group. The results showed that these bacteria included Bacillus sp. (including Bacillus subtilis and Bacillus
licheniformis) and Stenotrophomonas sp. (including Stenotrophomonas maltophilia and Stenotrophomonas pavanii) as
identified by 16S rDNA analysis, and included Clostridium sp. (including Clostridium perfringens) and Pseudomonas sp.
as identified by PCR-DGGE. It is speculated that the presence of Bacillus sp. and Clostridium sp. may be the major reason
for swollen bag that causes product deterioration.

Key words: pork jelly, swollen bag, 16S rDNA, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), Bacillus subtilis, Clostridium perfringens