FOOD SCIENCE

Previous Articles     Next Articles

Isolation and Identification of Two Endophytic Fungus Strains from Salicornia bigelovii

ZHAO Yu-hui,WANG Xiao-min,WANG Hui,PENG Jie,LIU Tian-xing,XIN Zhi-hong*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: XIN Zhi-hong

Abstract:

In this study, two strains of endophytic fungi were isolated and identified from Salicornia bigelovii collected
from the beach of Dafeng harbor, Yancheng city, Jiangsu province in China. The 18S rDNA and rDNA ITS1-5.8S-ITS4
regions of these strains were amplified by polymerase chain reaction (PCR) after the extraction of mycelial genomic
DNA and the amplification products were subjected to sequencing and homology analysis. A phylogenetic tree was
established based on the sequences of 18S rDNA and internal transcribed spacer (ITS) region, combined with morphological
observation. Therefore, both strains were identified as Phoma tracheiphila and Fusarium sp., respectively.

Key words: Salicornia bigelovii, morphology, 18S rDNA sequence, ITS1-5.8S-ITS4 sequence, phylogenetic analysis

CLC Number: