Abstract:

In this study, sly-3 strain that can inhibit Listeria monocytogenes was isolated from soil in Yantai suburb. The
morphological, physiological and biochemical characteristics of the isolated strain were basically consistent with those of
Bacillus subtilis and the homology of 16S rDNA sequence between sly-3 and B. subtilis CICC10034 was 99%. On the basis of
these results, sly-3 was finally identified as Bacillus subtilis. The antagonistic activity of sly-3 strain also suggested that only G+
bacteria could be inhibited but G－ bacteria could not be inhibited. Meanwhile, the 24 h culture filtrate of sly-3 exhibited high
inhibitory activity against Listeria monocytogenes from export aquatic products.

Key words: aquatic products, Listeria monocytogenes, antagonistic strain