Abstract:

Objective: To identify structurally the taxifolin from Larix olgensis Henry var. koreana Nakai and determinate
taxifolin content in the cross section of upper roots of Larix olgensis Henry var. koreana Nakai from Lijiang region of Jilin
province and in trees from 11 forest farms. Methods: Chromatographic separation was performed on a 15C18-AR waters column
(6 mm × 250 mm, 5 μm) with methanol/water (40:60, V/V) as the mobile phase at a flow rate of 1 mL/min. The column
temperature was 30 ℃. UV detection was performed at 288 nm. The injection volume was 20 μL. Results: The IR, UV, MS and
13C-NMR data obtained were consistent with those reported in the literature confirming the extract to be taxifolin. A good linear
relationship between peak area and taxifolin concentration in the range of 0.05 to 1.0 mg/mL was observed with correlation
coefficient r = 0. 9998. The average recovery of taxifolin was 99.3% with RSD of 2.6% (n = 9). In upper roots of Larix olgensis
Henry var. koreana Nakai, taxifolin was distributed in a gradually decreasing amount from pith to bark and also affected greatly
by growth conditions. Conclusion: The analytical method presented in this study proved simple, acurate, specific, repeatable
and applicable for quantitative analysis of taxifolin from Larix olgensis Henry var. koreana Nakai.

Key words: Larix olgensis Henry var. koreana Nakai, taxifolin, structural identification, high performance liquid chromatography (HPLC), content determination