Abstract:

In recent years, an increasing number of applications of probiotic dairy products have been reported to maintain
the intestinal flora balance, treat chronic and acute diarrhea and enhance the immune system. Nevertheless, many probiotic
products from the market are often not accurately labeled for the bacterial composition. Therefore, it is necessary to know
the bacterial composition of probiotic products for better judgment of their quality. For this purpose, we investigated the
accuracy and feasibility of 16S rDNA sequencing using Illumina next-generation sequencing (NGS) platform for bacterial
identification of the bacterial species composition in probiotic foods, especially in 24 probiotic products from 19 manufacturers.
Data showed that some probiotic products contained unadvertised lactobacilli and bifidobacteria, whereas others were
not the same as labeled. In summary, 16S rDNA sequencing by Illumina NGS platform is a rapid method for profiling the
microbial community in foods and clinical studies.

Key words: lactic acid bacterial, yoghurt, 16S rDNA, next-generation sequencing