Determination of Chondroitin Sulfate from Sea Cucumber by Methylene Blue Colorimetry

doi:10.7506/spkx1002-6630-20132249

Abstract

Abstract:

In this study, employing sea cucumber chondroitin sulfate standard samples extracted from 5 species of sea
cucumber, an efficient and rapid colorimetric method for determination of chondroitin sulfate from sea cucumber was established
based on the theory that methylene blue and anionic polymer can form supramolecular complex. The colorimetric
method was determined as follows: detection at 545 nm immediately following mixing of 1.00 mL of sea cucumber
chondroitin sulfate solution with 8.00 mL of 5 mmol/L pH 7.0 K2HPO4/KH2PO4 buffer and 1.00 mL of methylene blue
(0.18 mg/mL) and subsequent calculation of chondroitin sulfate concentration from the absorbance using the pre-prepared
calibration curve. This method proved to be simple, accurate and high-recovery and allow rapid quantitative determination
of sea cucumber chondroitin sulfate.

Key words: sea cucumber, sea cucumber chondroitin sulfate, methylene blue, colorimetry, determination

CLC Number:

TS207.3

XU Xiao-qi，XUE Chang-hu，ZHANG Cui-yu，YU Long，WANG Yan-chao，CHANG Yao-guang. Determination of Chondroitin Sulfate from Sea Cucumber by Methylene Blue Colorimetry[J]. FOOD SCIENCE, 2013, 34(22): 246-249.

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