Mutation of Saccharomyces cerevisiae Protoplasts for Enhanced Production of GSH and Gene Expression of GSH Over-accumulating Mutants

doi:10.7506/spkx1002-6630-201323037

FOOD SCIENCE

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Mutation of Saccharomyces cerevisiae Protoplasts for Enhanced Production of GSH and Gene Expression of GSH Over-accumulating Mutants

SUN Jiang1，ZHU Yi-bo2，WANG Li-mei2，DENG Da-qing2，QI Bin2,*

1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China；
2. Soochow Key Laboratory of Food Biotechnology, Research Centre of Fermentation Engineering Technology,
Changshu Institute of Technology, Changshu 215500, China

Online:2013-12-15 Published:2014-01-03
Contact: QI Bin

Abstract

Abstract:

In this study, Saccharomyces cerevisiae Y518 was improved for enhanced GSH production by protoplast
mutagenesis. The mortality of Saccharomyces cerevisiae protoplasts after being treated with 0.03 mol/L HNO2 for 3 min
was 85.96%. After 4 repeated treatments, a mutant was obtained which produced 14.85 mg of GSH per g dry weight, a 24%
increase over the original strain. The gene GSH1 encoding the rate-limiting enzyme in the synthesis route showed a 2.26-fold
increased expression, thus resulting in increased GSH production from the mutant strain.

Key words: Saccharomyces cerevisiae, protoplast mutagenesis, glutathione, gene GSH1

CLC Number:

Q815

SUN Jiang1，ZHU Yi-bo2，WANG Li-mei2，DENG Da-qing2，QI Bin2,*. Mutation of Saccharomyces cerevisiae Protoplasts for Enhanced Production of GSH and Gene Expression of GSH Over-accumulating Mutants[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201323037.

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Mutation of Saccharomyces cerevisiae Protoplasts for Enhanced Production of GSH and Gene Expression of GSH Over-accumulating Mutants

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