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Optimization of Medium Composition for Pigment Production by Fusarium sp. JN158 with Response Surface Methodology

WANG Yong-wei, LIAO Xiang-ru, LI Xun-hang, CAI Yu-jie*   

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology,
    Jiangnan University, Wuxi 214122, China
  • Online:2014-01-15 Published:2014-01-22
  • Contact: CAI Yu-jie

Abstract:

Plackett-Burman design and response surface methodology (RSM) were applied to optimize 7 components in the
medium for pigment production by Fusarium sp.JN158. The results showed that the yield of pigment was mainly influenced by
the concentrations of glucose, peanut meal and FeSO4·7H2O. With central composite design and RSM, the optimal medium
composition and concentrations was determined to be sucrose (30.18 g/L), peanut meal (4.86 g/L), K2HPO4·3H2O(1 g/L),NaCl
(0.5 g/L), MgSO4·7H2O (0.5 g/L), CaCl2 (0.5 g/L), and FeSO4·7H2O (0.0122 g/L). The experimental yield of pigment produced by
Fusarium sp. JN158 in the optimized medium was (1.252±0.011) g/L, which was close to the predicted value of 1.268 g/L.

Key words: Fusarium, pigment, Plackett-Burman design, response surface methodology, medium

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