Effect of Selenium-Modified Exopolysaccharide from Lactococcus lactis subsp. lactis on [Ca2+]i in Mouse Macrophages and Cancer Cells

doi:10.7506/spkx1002-6630-201401049

Abstract

Abstract:

Objective: The effect of Se-exopolysaccharide (EPS) from Lactococcus lactis subsp. lactis on intracellular free
Ca2+ levels in mouse macrophages cells, gastric cancer cells SGC7901 and Hela cells was studied. Methods: Cells labeled
with Fluo-3/AM dye for 10 min were examined using laser confocal scanning microscope. Results: The intracellular Ca2+
level in mouse macrophages increased in the presence of Se-EPS. On the other hand, Se-EPS could decrease the intracellular
Ca2+ levels both in gastric cancer cells SGC7901 and Hela cells. Conclusion: Se-EPS can activate macrophages, which may
be related to Ca2+ signal pathways. Se-rich EPS has gained the capacity to induceapoptosis in tumor cells.

Key words: exopolysaccharides, selenization, macrophage, Ca2+, cancer cells

CLC Number:

Q244
Q28

LIU Lu, PAN Dao-dong*, DING Lin, ZENG Xiao-qun. Effect of Selenium-Modified Exopolysaccharide from Lactococcus lactis subsp. lactis on [Ca2+]i in Mouse Macrophages and Cancer Cells[J]. FOOD SCIENCE, 2014, 35(1): 250-253.

References

[1] 王统一, 赵兵, 王玉春. 多糖免疫调节和抗肿瘤研究进展[J].过程工程学报, 2006, 6(4): 274- 268.

[2] 刘宇孟, 祥晨. 乳酸菌胞外多糖及其抗肿瘤活性[J]. 中国乳品业, 2008, 36(1): 39- 43.

[3] 顾笑梅, 王富生, 孔健等. 乳酸菌Z222产胞外多糖(EPSI)对免疫细胞功能的影响[J]. 中华微生物学和免疫学杂志, 2003, 23(6): 442- 445.

[4] 杨洁彬. 乳酸菌-生物学基础及应用[M]. 北京: 中国轻工业出版社, 1996.

[5] Rayman, M.P., The importance of selenium to human health. The Lancet, 2000, 356, 233-241.

[6] Guo, Y., Pan, D., Li, H., Sun, Y. et al., Antioxidant and immunomodulatory activity of selenium exopolysaccharide produced by Lactococcus lactis subsp. lactis. Food Chemistry, 2013, 138, 84-89.

[7] 胡庭俊, 程富胜, 陈炅然等.黄芪多糖对小鼠免疫细胞信号转导相关分子的影响[J].畜牧兽医学报, 2005, 36( 6) , 616- 619

[8] 程安玮, 金征宇, 万发春.甘草多糖对小鼠腹腔巨噬细胞NO、iNOS 及iNOS mRNA 表达的影响[J].食品科学.2009, 30(21): 351-354.

[9] 翟钦辉, 荣岳光, 董晓芳等.苜草素对巨噬细胞吞噬活性和NO、IL-6、TNF-α分泌的影响[J].营养报,2012,34(2): 181-185.

[10] 王鹏雁,安建多,王池.槲芪散及槲寄生提取物对肝癌细胞内游离钙离子浓度的影响[J].首都医科大学学报.2012, 33(3):291-296.

[11] Li Sun, Zhao Zhengyan, Zhou Xielai, Liu Suhang. The effect of lead on intracellular Ca2+ in mouse lymphocytes. Toxicology in Vitro 2008, 22, 1815-1819.

[12] 何建林,郑仕中,陆茵等.癌细胞中钙离子通道的研究进展[J].2012, 28(7):1027-1029.

[13] 王海燕, 何韶衡, 林珏龙等.激光扫描共聚焦显微镜观察胰蛋白酶对肺上皮细胞游离钙离子的影响[J]. 2005, 14(5):377-379

[14] 郭琼杰,孙淑.细胞内游离钙离子的测定方法食品与药品[J].2006, 8(12):25-27.

[15] 黄迪南,侯敢,祝其锋.猪苓多糖对小鼠腹腔巨噬细胞诱导型一氧化氮合酶活性及mRNA表达的影响[J].中西医结合学报,2004, (25): 350-352.