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Effects of Extraction Methods on Physico-chemical Properties of Rice Glutelin

LI Na, LI Xiang-hong, LIU Yong-le*, YU Jian, WANG Fa-xiang, WANG Jian-hui   

  1. Department of Food and Biology Engineering, Changsha University of Science and Technology, Changsha 410004, China
  • Online:2014-02-13 Published:2014-03-17

Abstract:

The effects of different extraction methods on physico-chemical properties of rice glutelin were studied. Sodium
dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE), size exclusion high performance liquid chromatography
(SEC-HPLC), scanning electron microscope (SEM) and differential scanning calorimetry (DSC) were used to characterize
the properties of extracted proteins. The results showed that the protein content of rice glutelin prepared by alkali extraction
combined with sequential extraction and washing process was (93.42±0.32)%. SDS-PAGE analysis revealed three clear
bands (19, 33 ku and 50 ku). The molecular weight distribution of the sample showed that there were fewer fractions with
small molecules and large molecules larger than 106 u. Our microstructural observation demonstrated that the protein
backbone structure was exposed after all four different alkali extractions. Meanwhile, the highest enthalpy of the sample
showed its good thermal stability.

Key words: rice glutelin, extraction, molecular structure