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Effect of Winemaking Conditions on β-Glucosidase Activity from Two Commercial Saccharomyces cerevisiae Strains

ZHANG Fang-fang, JIANG Lu, LIU Yan-lin   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China;
    2. Shaanxi Engineering Research Center for Viti-viniculture, Yangling 712100, China
  • Online:2014-02-13 Published:2014-03-17

Abstract:

The present study aimed to investigate the effect of vinification environments such as oxygen, pH, temperature,
sugar and ethanol on β-glucosidase activity from two commercial Saccharomyces cerevisiae strains. The results showed that
aerobic condition stimulated β-glucosidases biosynthesis. The optimal pH and temperature for β-glucosidase activity from
the whole cells of both strains were 5.0 and 60 ℃, respectively. β-Glucosidase activity was slightly inhibited by fructose,
glucose and sucrose. An ethanol concentration between 2% and 20% could activate β-glucosidase from the two S. cerevisiae
strains. During grape juice fermentation, the enzyme was mostly in both whole cells and permeabilized cells, while the
activity in the culture supernatant was low.

Key words: Saccharomyces cerevisiae, β-glucosidase, grape juice fermentation