Abstract:

Glycerol galactoside was synthesized from glycerol and galactose under the catalysis of β-galactosidase. Activated
carbon adsorption method was used to separate and purify the product. An L9 (34) orthogonal design based on singlefactor
experiments were employed to optimize the purification process. The obtained product was further purified by G-15
chromatography column. The optimized purification conditions were established as follows: 20-fold dilution of the reaction
product, activate carbon addition at a ratio of 2 g/mL and desorption with 30% ethanol. Under these conditions, the recovery
rate and purity of glycerol galactoside were 62.53% and 48.84%, respectively, which showed an increase of 4.52% and 4.76%,
respectively, over those obtained from single-factor experiments. The glycerol galactoside obtained from activated carbon
adsorption was further purified through Sephadex G-15 chromatography column, and its purity was increased to 97.80%. The
product was identified as glycerol monogalactoside by mass spectrometry (MS).

Key words: glycerol galactoside, separation and purification, orthogonal array experiments, activated carbon absorption