Abstract:

Fusarium sulphureum is a major pathogen causing dry rot of potato tubers, and has a strong ability to produce
mycotoxins. The incubation conditions for this pathogenic strain to produce mycotoxin in vitro were screened including
temperature, time, pH and stirring condition (static or shaking) and medium type. The results showed that the optimum
conditions for mycotoxin production were shaking culture at 25 ℃ for 10 days in Richard liquid medium at pH 4.5.
Temperature, pH and culture time were selected by using one-factor-at-a-time designs for further optimization by response
surface analysis using the average length of mung bean seed radicles as the response variable. A culture temperature of
22.2 ℃, pH 5.1 and 12 d culture were found optimal for mycotoxin production in vitro.

Key words: Fusarium sulphureum, crude toxin, response surface methodology, toxin production condition