Abstract:

A liquid chromatography-mass spectrometry (LC-MS) method was developed for the determination of micro
amounts of fructose and glucose in beers. In this method, samples were detected directly after filtering. The chromatographic
separation was achieved on a Waters Acquity column (2.1 mm×150 mm, 1.7 μm), with a mobile phase composed of water
and acetonitrile (10:90, V/V). The mass spectrometer was operated in the negative mode using MRM．The developed
calibration curves displayed good linearity over a concentration range of 1.25 to 30 mg/L with a correlation coefficient equal
to 1 for fructose, and 2 to 50 mg/L with a correlation coefficient equal to 0.999 9 for glucose. The limits of detection (LOD)
were 0.05 and 0.49 mg/L respectively. This method has been successfully used for the determination of fructose and glucose
in beers with satisfactory results.

Key words: beers, fructose, glucose, liquid chromatography-tandem mass spectrometry (LC-MS)