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Decolorization, Demineralization and Monosaccharide Composition of Xylooligosaccharides from Sugarcane Bagasse

SHENG Jin-feng1,2, LI Li1,2, SUN Jian1,2,3,*, LI Chang-bao1,2, ZHAO Mou-ming3, HE Xue-mei1,2, ZEHNG Feng-jin1,2, LI Jie-min1,2, LIU Guo-ming1,2, LIAO Fen1,2   

  1. 1. Institute of Agro-Food Science & Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
    2. Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Nanning 530007, China;
    3. College of Light Industry and Food, South China University of Technology, Guangzhou 510640, China
  • Online:2014-07-25 Published:2014-08-26
  • Contact: SUN Jian

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Abstract:

Decolorization and demineralization of xylooligosaccharides (XOs) from sugarcane bagasse were investigated
by activated charcoal combined with ion exchange resin adsorption. The monosaccharide composition of XOs was analyzed
by high performance liquid chromatography (HPLC). Results showed that the optimum decolorization conditions for
XOs were addition of 1% activated charcoal followed by adsorption at 60 ℃ for 1 h, resulting in a decolorization rate of
80.25% and a retention rate of reducing sugar of 98.7%. Through a comparative analysis of seven different types of resins,
sequential chromatography on 001×7 and D301 with a volume ratio of 2:1 at a flow rate of 254 mL/h was chosen for
the best demineralization of XOs. The decolorized and demineralized XOs exhibited a decolorization rate of 92.4% and a
demineralization rate of 79.2%, and were nearly neutral (pH 7.4). HPLC analysis showed that the major monosaccharide
from acid hydrolysates of purified XOs, accounting for 88.9% of the total monosaccharides, and small amounts of mannose
and glucose were detected as well. The XOs obtained in this study mainly contained xylobiose and xylotriose, together with
a small amount of xylose and xylopentaose.

Key words: xylooligosacchardies, activated charcoal, ion exchange resin, decolorization and demineralization, monosaccharide composition

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