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Simultaneous Screening for 14 Mycotoxin Contaminants in Foods by QuEChERS-LC-MS-MS

SHI Na1,2, HOU Cai-yun1,*, LU Yong2, JIANG Jie2, ZHANG Xue-liang3   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
    2. Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China;
    3. College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
  • Online:2014-08-25 Published:2014-08-25
  • Contact: HOU Cai-yun

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Abstract:

This paper presents a QuEChERS-liquid chromatography-tandem mass chromatography (LC-MS-MS) method
for the simultaneous screening for 14 mycotoxin contaminants (deoxynivalenol, penicillic acid, aflatoxin M1, aflatoxin G2,
citrinin, aflatoxin B1, aflatoxin B2, aflatoxin G1, zearalenone, ochratoxin A, sterigmatocystin, HT-2 toxin, T-2 toxin and
podophyllotoxin) in foods. The homogenized samples were extracted with 1% acetic acid in acetonitrile. The separation
was performed on an ACQUITU UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) by gradient elution after purification
by dispersive-solid-phase extraction (d-SPE). The 14 mycotoxin contaminants in foods analyzed by LC-MS-MS-ESI in
the positive ionization, multiple-reaction monitoring (MRM) mode. The minimum detection limit was 0.5–1 μg/kg. The
developed method proved to be simple, rapid, highly selective and sensitive, and suitable for the analysis of mycotoxin
contaminants in food safety incidents.

Key words: mycotoxin contaminants, QuEChERS, liquid chromatography-tandem mass spectrometry (LC-MS-MS), screening, food

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