Abstract:

A new method was developed for the determination of vitamin C in foods by ultra performance convergence
chromatography (UPC2). The mobile phase was a mixture of supercritical CO2 and methanol (containing 0.05% H3PO4) at a
flow rate of 0.6 mL/min. A Waters CSH Fluoro-Phenyl column (3.0 mm × 100 mm, 1.7 μm) was used, and the UV detector
was set at 245 nm. The limit of detection (LOD) was 1.5 mg/kg, and the calibration linear range was between 5 and 200 mg/L.
The spiked recoveries of vitamin C were 96.05%–101.15%, with relative standard deviation (RSD) ranging from 0.52% to
0.76%. The method is efficient, rapid, simple, sensitive, accurate, repeatable and low-cost with a low limit of detection. It
can be applied for the determination of vitamin C in foods.

Key words: ultra performance convergence chromatography (UPC2), vitamin C, determination, food