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Recent Progress in Application of Loop-Mediated Isothermal Amplification Method for Detection of Enterobacteriaceae Pathogens

HE Xiao-hua, DUN Yu-hui, LU Li, LI Ke, LIU Bin   

  1. 1. College of Plant Protection, Northwest A&F University, Yangling 712100, China;
    2. Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou 310016, China;
    3. Yiwu Academy of Science & Technology for Inspection & Quarantine, Yiwu 322000, China;
    4. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2014-10-15 Published:2014-10-17

Abstract:

In recent years, loop-mediated isothermal amplification (LAMP) has emerged as a new technology for nucleic
acid amplification under constant temperature conditions, which is characterized by simple instrumental requirements,
rapid detection, high sensitivity, high specificity, and efficient amplification. This technology has been widely applied in
the detection of foodborne pathogens. This paper focuses on the recent progress in the application of LAMP assay in the
detection of common Enterobacteriaceae pathogens.

Key words: loop-mediated isothermal amplification (LAMP), Enterobacteriaceae, pathogens, detection

CLC Number: