Abstract:

Objective: A loop-mediated isothermal amplification (LAMP) method was established for the detection of
mustard allergen in foods and was compared with real-time polymerase chain reaction (RT-PCR). Methods: The Sin A1 gene
of Sinapis alba was used to design LAMP primers. The specificity and sensitivity of LAMP were compared with those of
RT-PCR. Results: The specificity of LAMP method was tested with 13 different crops. The results showed that the LAMP
method was highly specific to mustard. No cross-reaction was found. The limit of detection (LOD) for LAMP was 0.5%
in base-material addition test, which was consistent with that of the RT-PCR method. For 25 real food samples, the LAMP
results were consistent with the RT-PCR results. Conclusion: The LAMP method of established in this study was simple,
economical and reliable and could effectively reduce the detection time.

Key words: mustard, allergen, loop-mediated isothermal amplification (LAMP), real-time polymerase chain reaction (RT-PCR)