Abstract:

This study aimed to investigate the in vivo anti-inflammatory activity of trilobatin extracted from the young leaves
of Lithocarpus polystachyus Rehd on lipopolysaccharide (LPS)-induced inflammatory response in mice. After being treated
intragastrically with trilobatin at three different dosages (3, 30 and 300 mg/(kg·d)) for 3 days, the mice were administered
intraperitoneally with LPS (0.1 mg/kg) 1 h after the last intragastrical treatment on day 3. The effect of trilobatin on the
expression of LPS-induced pro-inflammatory cytokines was detected. Tumor necrosis factor-α (TNF-α) and interleukin-6
(IL-6) in serum were measured using enzyme-linked immunosorbent assay (ELISA) and the mRNA expression levels of
TNF-α and IL-6 in liver were assayed with real-time quantitative polymerase chain reaction (RT-qPCR). The protein levels
of TNF-α and IL-6 in liver were detected by immunohistochemical strept avidin-biotin complex (SABC) method. We found
that trilobatin at low dosage (3 mg/(kg·d)) significantly inhibited LPS-induced TNF-α and IL-6 production in serum, and
decreased their expression in liver. These results indicate that trilobatin from Lithocarpus polystachyus Rehd has protective
effect on inflammatory response in mice.

Key words: Lithocarpus polystachyus Rehd, trilobatin, anti-inflammation, lipopolysaccharide (LPS), inflammatory response