Screening of Yeast Strains Producing β-Glucosidase from Hexi Corridor Wine-Producing Regions of Gansu Province

doi:10.7506/spkx1002-6630-201423028

Abstract

Abstract:

Totally 115 yeast strains isolated from spontaneously fermented ripe grape berries from Hexi Corridor wineproducing
regions of Gansu province were subjected to two-round screening on plates followed by in shaking flasks using
p-nitrophenyl-β-D-glucopyranoside (p-NPG) as a substrate for β-glucosidase activity. The strains with higher β-glucosidase
activity were subjected to taxonomic identification using WL nutrient medium and lysine medium, and sequence analysis of
the 26S rDNA D1/D2 domain. Six yeast isolates with β-glucosidase activity similar to that of commercial yeast ICV-D254,
(51.40±4.74) mU/mL, were obtained. Strains QLFE, MQFEH-1, MQFSC-3, MQFEH-2 and MQFSM-3 were identified as
Saccharomyces cerevisiae, and QLFE-4 as Metschnikowia. These identification results were in accordance with those from
sequence analysis of the 26S rDNA D1/D2 domain.

Key words: yeast screening, β-glucosidase activity, identification, sequence analysis of the 26S rDNA D1/D2 domain

CLC Number:

TS261.1

HOU Xiao-rui1,2,3, WANG Jing1,2,3, YANG Xue-shan4, SHENG Wen-jun1,2,3, ZHU Xia1,2,3, HAN Shun-yu1,2,3,*. Screening of Yeast Strains Producing β-Glucosidase from Hexi Corridor Wine-Producing Regions of Gansu Province[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201423028.

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Screening of Yeast Strains Producing β-Glucosidase from Hexi Corridor Wine-Producing Regions of Gansu Province

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