Abstract:

This work was executed to explore the best debittering process for rice protein hydrolysate by separately using
flavourzyme, active carbon and β-cyclodextrin. The debittering efficiency was measured by means of electronic tongue (ET)
and the angiotensin Ⅰ-converting enzyme (ACE) inhibitory activity of rice protein hydrolysate before and after debittering
was evaluated by high performance liquid chromatography (HPLC). Results showed that the optimal debittering conditions
were hydrolysis at an initial pH of 5.8 for 20 min with 2.5% of flavourzyme, treatment at 60 ℃ for 20 min with 2.0% of
active carbon or embedding for 30 min with 10% of β-cyclodextrin.

Key words: ACE inhibitory peptides, debittering, electronic tongue, flavourzyme, active carbon, β-cyclodextrin