Abstract:

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination
of 11 functional components in health foods. Samples were extracted with methanol-water-phosphoric acid solution/
n-hexane-methanol-water-phosphoric acid solution (for lipid-containing samples). Analysis was performed using an HPLC
system equipped with an Agilent TC- C18 column (250 mm × 4.6 mm, 5 μm) and a PDA/FLR detector with a mobile phase
composed of acetonitrile and 2 g/L sodium octanesulfonate solution (containing 1 mL of phosphoric acid) at a flow rate of
1.0 mL/min with gradient elution. Good linearity was observed in the range of 0.25–25 mg/L for vitamin B2, 0.5–20 mg/L
for vitamin B6, 1–100 mg/L for nicotinic acid and nicotinamide, 0.5–50 mg/L of the other five functional components with
correlation coefficients of 0.999 1–1.000 0. The average recoveries in health foods were in the range of 86.4%–110% at three
spiked levels with relative standard deviations varying between 0.21% and 11.7% (n = 6). The limit of quantification was
20 mg/kg for nicotinic acid and nicotinamide, and 10 mg/kg for the remaining nine functional components.

Key words: high performance liquid chromatography (HPLC), water-soluble vitamin, chromium picolinate, melatonine, salidroside