Abstract:

Purpose: To prepare and characterize Ganoderma lucidum triterpenoids-bovine serum albumin (BSA) nanoparticles.
Methods: Ganoderma lucidum triterpenoids-BSA nanoparticles were prepared by desolvation-chemical crosslinking
method and the preparation conditions were optimized with respect to entrapment efficiency by orthogonal array design.
Results: Nanoparticles with an average particle size of (189 ± 11.13) nm, a polydispersity index (PDI) of 0.227 ± 0.016,
a zeta potential of (−31.4 ± 6.74) mV, an entrapment efficiency of (80.10 ± 1.05)%, and a drug-loading efficiency of
(11.36 ± 0.13)% were obtained by dropwise addition of 8 mg of Ganoderma lucidum triterpenoids dissolved in 25 mL of
absolute ethanol to 50 mg/mL aqueous solution of BSA while constantly stirring the mixture at a speed of 400 r/min and
subsequent prompt addition of 125 μL of 0.25% glutaraldehyde. The nanoparticles were stable at 4 ℃ for 30 d.

Key words: Ganoderma lucidum triterpenoids, bovine serum albumin, nanoparticles, stability