Abstract:

In order to improve the purity of polysaccharides from the pomace of wild Crataegus songorica, NKA-9 and D101
were selected from 7 kinds of macroporous resins by static adsorption-desorption experiments, and the two selected resins
were mixed at an appropriate ratio. On the basis of single factor experiments, response surface methodology was used to
optimize process parameters. The optimal mixing ratio of NKA-9 to D101-1 was 2:3 (by mass). The optimum experimental
parameters for adsorption were as follows: sample loading flow rate, 3.75 mL/min; sample concentration, 1.32 g/L; and resin
diameter-to-height ratio, 1:13, yielding a rate of adsorption of 60.75%. Under the optimal desorption conditions: 7 BV of
0.27 mol/L NaCl as eluent at a f??l ow rate of 3.5 mL/min, a rate of desorption of 84.22% could be achieved. The content of
polysaccharides was increased from 5.06% to 21.13%.

Key words: Crataegus songorica, polysaccharides, response surface methodology, purification