Abstract:

Volatile metabolites from Staphylococcus aureus cultured with tryptone soy broth (TSB) were extracted under
different conditions namely three types of fiber (65 μm PDMS/DVB fiber, 75 μm CAR/PDMS fiber and 50/30 μm DVB/
CAR/PDMS fiber), three extraction times (20, 30 and 40 min) and three extraction temperatures (50, 65 and 80 ℃),
respectively. The extracts were detected by gas chromatography-mass spectroscopy (GC-MS). The optimal extraction
conditions were determined. The results indicated that 50/30 μm DVB/CAR/PDMS fiber was suitable for the extraction of
various components and more comprehensive information was obtained using this fiber. Abundant amounts and response
intensities of bacterial volatile metabolites were extracted at 80 ℃ for 30 min. Accordingly the optimal extraction conditions
for volatile metabolites from S. aureus were obtained. Using the established extraction method, the main volatile compounds
produced by S. aureus cultured in TSB were detected to be alcohol, 1-decanol, 3-hydroxy-2-butanone, acetone, acetic acid,
benzyl alcohol, 3-methyl-1-butanol, indole, 1-octanol and octanoic acid. This extraction method can provide a valuable tool
for the analysis of bacterial volatile metabolites.

Key words: Staphylococcus aureus, volatile metabolites, extraction condition, optimization, headspace solid phase micro-extraction gas chromatography-mass spectroscopy (HS-SPME-GC-MS)