Immunomagnetic Capture PCR for Rapid Detection of Listeria monocytogenes

doi:10.7506/spkx1002-6630-201512043

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Immunomagnetic Capture PCR for Rapid Detection of Listeria monocytogenes

LI Jingheng1, GUO Huiqin2, GAO Xiaoqiang3, LIU Fang3, QIU Shi1, LI Jianwu1, ZENG Haijuan1, LIU Lan2, SONG Chunmei1, LIU Qing1,*

1. College of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology,
Shanghai 200093, China; 2. Prajna Biology Technique Co. Ltd., Shanghai 200433, China;
3. Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730020, China

Online:2015-06-25 Published:2015-06-12
Contact: LIU Qing

Abstract

Abstract:

This experiment was conducted using immunomagnetic enrichment followed by amplification with polymerase
chain reaction (PCR) for rapid detection of Listeria monocytogenes. The best coating conditions were achieved by shaking
the prepared immunomagnetic beads at 37 ℃ for 1 h, and the amount of antibody conjugated to the immunomagnetic beads
was 100 μg/mg, leading to a capture rate of 45%. The immunomagnetic capture PCR assay was able to detect as low as
104 CFU/mL Listeria monocytogenes with 10 times higher sensitivity than direct PCR showing a limit of detection of
105 CFU/mL. This PCR assay can provide a new method for the enrichment and rapid detection of pathogenic bacteria.

Key words: IMBs, Listeria monocytogenes, PCR

CLC Number:

TS201.3

LI Jingheng1, GUO Huiqin2, GAO Xiaoqiang3, LIU Fang3, QIU Shi1, LI Jianwu1, ZENG Haijuan1, LIU Lan2, SONG Chunmei1, LIU Qing1,*. Immunomagnetic Capture PCR for Rapid Detection of Listeria monocytogenes[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201512043.

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Immunomagnetic Capture PCR for Rapid Detection of Listeria monocytogenes

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