Abstract:

The aim of this work was to optimize the enzymatic hydrolysis of carrot pulp with cellulase and pectinase for
enhanced juice yield by response surface methodology (RSM). The one-factor-at-a-time method was adopted to investigate
the effect of cellulase to pectinase ratio, hydrolysis temperature and time on juice yield. The interactions of the three
independent variables were analyzed using Box-Behnken design and response surface analysis. As a result, a quadratic
polynomial regression model was established. The optimal hydrolysis conditions for producing carrot juice were found to
be enzymatic hydrolysis with 0.3% of a mixture of cellulase and pectinase (2.2:10, g/g) for 1.94 h at 41.67 ℃. Experiments
carried out under these conditions gave a juice yield of (79.36 ± 0.23)%, was agreeing wit the predicted value (79.06%).

Key words: carrot, mixed enzymes, enzymatic hydrolysis, juice yield, response surface methodology