Abstract:

The enzymatic synthesis of palmitoyl-monogalactosylglycerol from monogalactosyl glycerol and free palmitic
acid in organic solvent was investigated in present study. Novozyme 435, an immobilized lipase, was used as catalyst.
The main factors that influence the yield of palmitoyl-monogalactosylglycerol, including lipase amount, and molar
ratio of monogalactosyl glycerol to palmitic acid and reaction time, were selected and optimized by the combined use
of single factor experiments and response surface methodology. The optimal synthesis conditions were determined as
follows: reaction solvent, acetone; Novozyme 435 amount, 12.93 mg/mL; molar ratio monogalactosylglycero to palmitic
acid, 1.00:4.49; reaction temperature, 55 ℃; and reaction time, 27.60 h. Under these conditions, the yield of palmitoylmonogalactosylglycerol
was 90.17%.

Key words: palmitoyl-monogalactosylglycerol, enzymatic synthesis, acetone, Novozyme 435, optimization